Kirby-Bauer Test (Disk Diffusion Test)
The Kirby-Bauer Test (disk-diffusion test) is a technology to test the effectiveness of antibiotics on a specific micro-organism.
- Description
| Testing Method | Kirby-Bauer Test (Disk Diffusion Test) |
| Description | The disk diffusion test, or agar diffusion test, or Kirby–Bauer test (disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test, KB test), is a test of the antibiotic sensitivity of bacteria. It uses antibiotic discs to test the extent to which bacteria are affected by those antibiotics. In this test, wafers containing antibiotics are placed on an agar plate where bacteria have been placed, and the plate is left to incubate. If an antibiotic stops the bacteria from growing or kills the bacteria, there will be an area around the wafer where the bacteria have not grown enough to be visible. This is called a zone of inhibition.
A pure bacterial culture is suspended in buffer, standardized to turbidity and swabbed uniformly across a culture plate. A filter-paper disk, impregnated with the compound to be tested, is then placed on the surface of the agar. The compound diffuses from the filter paper into the agar. The concentration of the compound will be highest next to the disk, and will decrease as distance from the disk increases. If the compound is effective against bacteria at a certain concentration, no colonies will grow where the concentration in the agar is greater than or equal to the effective concentration. This is the zone of inhibition. This along with the rate of antibiotic diffusion are used to estimate the bacteria’s susceptibility to that particular antibiotic. In general, larger zones correlate with smaller minimum inhibitory concentration (MIC) of antibiotic for that bacteria. Inhibition produced by the test is compared with that produced by known concentration of a reference compound. This information can be used to choose appropriate antibiotics to combat a particular infection. |
| More Information | Wikipedia: Disk Diffusion Test |
Related Products
-
Particle Size Analyzer
Particle size analyzer provides accurate, reliable particle size distribution measurements from nanometers to millimetres.
-
Shaking Incubator
Shaking incubator is often used for cell culturing, cell aeration, and solubility studies.
-
Mass Spectrometry (Q-TOF-MS)
Mass spectrometry (Q-TOF-MS) is an analytical technique that ionizes chemical species and sorts the ions based on their mass to charge ratio.
-
Scanning Electron Microscope (SEM + EDX)
Scanning electron microscopy (SEM) is a non-destructive technique that uses an electron beam probe to analyse surface details down to nano-scale, and to produce high magnification images with high resolution.
-
Mercury Intrusion Porosimetry (MIP)
Mercury intrusion porosimetry (MIP) is used to evaluate porosity, pore size distribution, and pore volume of various solid and powder materials.
